Datasets
Visualization
Raw data were converted from Zeiss CZI files to VTK files. VTK files can be open with Fiji and the BioEmergences's Fiji Tools available here. Alternatively, Paraview can natively display VTK files.
Wild type specimens
140423aX From 16 hpf to 26 hpf 44min
( 230 timesteps; imaging interval Δt=4 min; corrected for 28.5°C developmental time to Δt=2.8 min)
Transgene: Tg[cldnb:lynGFP] Tg[Brn3c:GFP]
Staining: RNA injection 1-cell stage for nuclear staining (H2B-mCherry)
Volume: 510 x 510 x 201 voxels
Voxel size: 0.31 µm x 0.31 µm x 0.46 µm
140426aX From 18 hpf to 36 hpf 12min
( 390 timesteps; imaging interval Δt=4 min ; corrected for 28.5°C developmental time to Δt=2.8 min)
Transgene: Tg[cldnb:lynGFP] Tg[Isl3:GFP]
Staining: RNA injection 1-cell stage for nuclear staining (H2B-mCherry)
Volume: 449 x 449 x 280 voxels
Voxel size: 0.37 µm x 0.37 µm x 0.47 µm
140507aX From 24 hpf to 43 hpf
( 326 timesteps; imaging interval Δt=5 min ; corrected for 28.5°C developmental time to Δt=3.5 min)
Transgene: Tg[Brn3c:GFP]
Staining: RNA injection 1-cell stage for nuclear staining (H2B-mCherry)
Volume: 551 x 551 x 200 voxels
Voxel size: 0.31 µm x 0.31 µm x 0.47 µm
Morphant
140519aX From 24 hpf to 42 hpf 15min
( 313 timesteps; imaging interval Δt=5 min; corrected for 28.5°C developmental time to Δt=3.5 min)
Transgene: Tg[Brn3c:GFP]
Staining: RNA injection 1-cell stage for nuclear staining (H2B-mCherry)
Manipulation: Injection 1-cell stage of MO-neurog1
Volume: 441 x 690 x 231 voxels
Voxel size: 0.31 µm x 0.31 µm x 0.47 µm